ABSTRACT
Salvia Plebeia is the full grass of Salvia Plebeia R.Br.,which contains chemical constituents belonging to flavonoids, terpenoids, phenolic acids, volatile oils, sterols and other ingredients. The pharmacological effects include relieving cough and asthma, anti-inflammatory, analgesia antioxidant, hepatoprotective, antibacterial and other activities. It is commonly used to treat herpes zosters, hemorrhoids, children with acute nephritis, bronchitis and so on in clinic. This article summarized the research on chemical constituents, pharmacological effects and clinical application in recent years, in order to provide references for further research and development of Salvia Plebeia.
ABSTRACT
BACKGROUND: Asthma is an increasing global health problem, and novel strategies to prevent or ameliorate the condition are needed. Here, the effects of 80 % ethanol extracts of Salvia plebeia R. Br. (SE) on an induced inflammatory response were investigated RESULTS: Salvia plebeia R. Br. inhibited production of pro-inflammatory cytokines, such as TNF-α and IL-6, as well as nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. NO and pro-inflammatory cytokine production was suppressed more effectively by SE of the aerial parts (SE-A) than of the roots (SE-R) of S. plebeia. In BEAS-2B cells, both SE-A and SE-R inhibited the increase in production of the inflammatory cytokines IL-6 and IL-8. We also investigated the antiasthmatic effects of SE in an ovalbumin (OVA)-induced BALB/c mouse model. SE-A treatment significantly reduced the number of airway eosinophils, IL-4 and IL-13 levels, mucus production, and inflammatory infiltration, as compared with the corresponding levels in the untreated, OVA-induced mice, and had similar effects to dexamethasone CONCLUSIONS: Salvia plebeia ethanol extract ameliorated the induced inflammatory response in RAW 264.7 and BEAS-2B cells, with more effective inhibition noted for SE-A than for SE-R. SE-A treatment was effective in improving the histopathological changes in the lungs of asthma model mice via modulation of eosinophils and Th2 cytokines. These results suggest that SE-A can be considered as a therapeutic agent that can potentially relieve asthma
Subject(s)
Animals , Female , Mice , Asthma/drug therapy , Drugs, Chinese Herbal/pharmacology , Anti-Asthmatic Agents/pharmacology , Anti-Inflammatory Agents/pharmacology , Asthma/chemically induced , Enzyme-Linked Immunosorbent Assay , Cells, Cultured , Ovalbumin , Lipopolysaccharides/pharmacology , Reproducibility of Results , Cytokines/analysis , Cytokines/drug effects , Plant Components, Aerial/chemistry , Disease Models, Animal , Ethanol/pharmacology , Real-Time Polymerase Chain Reaction , RAW 264.7 Cells , Lung/drug effects , Lung/physiology , Mice, Inbred BALB C , Nitric Oxide/analysisABSTRACT
Objective:To develop an HPLC method for the simultaneous determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins. Methods:The column was Agilent-C18 (250 mm × 4. 6 mm,5μm) with the temperature of 25℃, the mobile phase was methanol-water-glacial acetic acid-triethylamine (90 ∶10 ∶0. 03∶0. 06) at the flow rate of 1. 0 ml·min-1 ,and the detection wavelength was 210 nm. Results: The calibration curves were linear within the range of 7. 50-75. 00 μg · ml-1 for oleanic acid(r=0. 999 7)and 5. 00-50. 00 μg · ml-1 for ursolic acid(r=0. 999 7). The average recovery of oleanic acid and ursolic acid was 100. 15%(RSD=1. 14%,n=6)and 99. 40%(RSD=1. 67%,n=6), respectively. Conclusion: The method is simple and accurate, which is suitable for the determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins.
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Objective: To study the chemical constituents from the herb of Salvia plebeian. Methods: The constituents were isolated and purified by silica gel, Sephadex LH-20, and ODS column chromatography, and preparative TLC. The structures of the isolated compounds were elucidated by spectroscopic analysis and physicochemical properties. Results: Eleven compounds were isolated and purified from the ethanol extract of S. plebeia. They were identified as 5,6-dihydroxy-7,4'-dimethoxyflavone (1), 5,6,3'-trihydroxy-7,4'-dimethoxyflavone (2), nepetin (3), homoplantaginin (4), apigenin (5), cirsiliol (6), 6-methoxynaringenin (7), emodin (8), maslinic acid (9), isorosmanol (10), and (-)-epiloliolide (11). Conclusion: Among them, compound 11 is isolated from the plants of Salvia L. firstly, and compounds 1, 2, 6, 9, and 10 are isolated from S. plebeian for the first time.
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OBJECTIVE: To study the chemical constituents of the roots of Salvia plebeia R.Br.
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Objective To study the antitumor activity of extract from Salvia plebeia and investigate whether the extract induce apoptosis of K562 cells. Methods The aqueous, petroleum ether, dichloromethane (CH2Cl2), ethyl acetate, and butanol extracts were prepared from the aerial parts of 5. plebeia. Taking fluorouracil as reference, the cytotoxic activities of these extracts on HeLa, A549, SGC-7901, HCT-116, K562, LoVo, DU-145, and HepG2 cells were evaluated. To clarify the apoptosis of K562 cells induced by CH2Cl2 extract, the methods of Hoechst 33258 staining, flow cytometry assay, and DNA ladder assay were investigated. Results The CH2Cl2 extract showed the most potent cytotoxic effect against K562 cells, with an IC50 < 15 μg/mL for 3 d treatment. The characteristic apoptotic symptoms such as DNA fragmentation and chromatin condensation were also observed in the K562 cells. Conclusion The CH2Cl2 extract from S. plebeia may inhibit the cancer cell proliferation by inducing cell apoptosis.